Journal:

Article Title: Peptide Nucleic Acid Antisense Oligomer as a Therapeutic Strategy against Bacterial Infection: Proof of Principle Using Mouse Intraperitoneal Infection

doi: 10.1128/AAC.49.8.3203-3207.2005

Figure Lengend Snippet: Inhibition of E. coli mutant SM101 growth in vitro by acpP-targeting PNA. PNA was added to cell cultures containing 5 × 105 CFU/ml SM101 to a final concentration of 4, 40, or 400 μM as indicated on the graph, with addition of an equal volume of water as a control (0 μM). Cultures were incubated as described in Materials and Methods. Aliquots of each culture were collected at 2 and 4 h, diluted, and plated for viable cell determination. Error bars indicate standard deviations of the results from three experiments (*, P < 0.01 relative to 2 h control; **, P < 0.001 relative to 4-h control [as determined by Student's t test]).

Article Snippet: E. coli strain K-12 was obtained from the American Type Culture Collection (ATCC 29425) and grown at 37°C in nutrient broth.

Techniques: Inhibition, Mutagenesis, In Vitro, Concentration Assay, Incubation

Journal:

Article Title: Peptide Nucleic Acid Antisense Oligomer as a Therapeutic Strategy against Bacterial Infection: Proof of Principle Using Mouse Intraperitoneal Infection

doi: 10.1128/AAC.49.8.3203-3207.2005

Figure Lengend Snippet: Inhibition of E. coli wild-type K-12 growth in vitro by acpP-targeting peptide-PNA conjugate. Peptide-PNA conjugate was added to cell cultures containing 5 × 105 CFU/ml E. coli strain K-12 to a final concentration of 0, 4, 40, or 400 μM as indicated on the graph. Cell cultures were grown, and viable cells were determined as described in the legend to Fig. ​Fig.1.1. Error bars indicate standard deviations of the results from two experiments (*, P < 0.001 relative to 2-h control; **, P < 0.005 relative to 4-h control [as determined by Student's t test]).

Article Snippet: E. coli strain K-12 was obtained from the American Type Culture Collection (ATCC 29425) and grown at 37°C in nutrient broth.

Techniques: Inhibition, In Vitro, Concentration Assay

Journal:

Article Title: Peptide Nucleic Acid Antisense Oligomer as a Therapeutic Strategy against Bacterial Infection: Proof of Principle Using Mouse Intraperitoneal Infection

doi: 10.1128/AAC.49.8.3203-3207.2005

Figure Lengend Snippet: Survival of K-12-infected mice due to treatment with acpP-targeting peptide-PNA conjugate. Six-week-old BALB/c mice were i.p. infected with 8 × 108 CFU E. coli strain K-12 in 500 μl PBS (LD90). Infected mice were treated with the peptide-PNA conjugate (100 or 500 nmol) 30 min postinfection; comparisons are made to untreated mice. Survival and general health of these animals was monitored every 6 to 12 h through 9 days.

Article Snippet: E. coli strain K-12 was obtained from the American Type Culture Collection (ATCC 29425) and grown at 37°C in nutrient broth.

Techniques: Infection

Journal:

Article Title: Peptide Nucleic Acid Antisense Oligomer as a Therapeutic Strategy against Bacterial Infection: Proof of Principle Using Mouse Intraperitoneal Infection

doi: 10.1128/AAC.49.8.3203-3207.2005

Figure Lengend Snippet: Reduction of E. coli K-12 bacteremia in mice following treatment with peptide-PNA conjugate a

Article Snippet: E. coli strain K-12 was obtained from the American Type Culture Collection (ATCC 29425) and grown at 37°C in nutrient broth.

Techniques: